Integrando ADN y taxonomía morfológica para describir una nueva especie de la familia Bathynellidae (Crustacea, Syncarida) de España

A. I. Camacho; B. A. Dorda; I. Rey

doi:10.3989/graellsia.2013.v69.086

Authors

A. I. Camacho Museo Nacional de Ciencias Naturales (CSIC)
B. A. Dorda Museo Nacional de Ciencias Naturales (CSIC). Dpto. Colecciones, Colección de Tejidos y ADN
I. Rey Museo Nacional de Ciencias Naturales (CSIC). Dpto. Colecciones, Colección de Tejidos y ADN

DOI:

https://doi.org/10.3989/graellsia.2013.v69.086

Keywords:

Syncarida, Bathynellacea, Bathynellidae, Spain, groundwater fauna, new species, morphological data, mtDNA, COI, nuclearDNA, 18S

Abstract

A new species of Bathynellidae Grobben, 1905 is described from Spain. Vejdovskybathy-nella vasconica sp. nov. displays an exclusive feature within the genus: eightsegmented antenna. Besides, the new species has a unique combination of morphological characters, including medial seta on exopod of antenna, antennule length similar to antenna, three-segmented mandibular palp without sexual dimorphism, four segments on endopod of thoracopod I to VII, three spines on the sympod of uropod, two claws on the endopod of uropod, first spine longer than the rest on the furcal rami, exopod smaller than endopod on female thoracopod VIII, a long frontal projection and medium size outer protuberance on penial region of male thoracopod VIII, and a medium size frontal crest with a small “spur” on basipod of male thoracopod VIII. Partial sequences from mitochondrial gene cytochrome oxidase I (COI) and 18S ribosomal RNA (rRNA) gene have been obtained from specimens of the type locality of the new species. The analyses of molecular data demonstrate the presence of two highly divergent genetic units within the Bathynellidae, corresponding to two morphologically well differenciated genera.

Downloads

Download data is not yet available.

References

Abrams, K. M., Guzik, M. T, Cooper, S. J. B., Humphreys, W. F., King, R. A., Cho, J.-L. & Austin, A. D., 2012. What lies beneath: Molecular phylogenetics and ancestral state reconstruction of the ancient subterranean Australian Parabathynellidae (Syncarida, Crustacea). Molecular Phylogenetics and Evolution, 64: 130-164. http://dx.doi.org/10.1016/j.ympev.2012.03.010

Altschul, S. F., Madden, T. L., Schaeffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25: 3389-3402. http://dx.doi.org/10.1093/nar/25.17.3389

Camacho, A. I., 2006. An annotated checklist of Syncarida (Crustacea, Malacostraca) in the world. Zootaxa, 1374: 1-54.

Camacho, A. I., 2007. The first record of the genus Vejdovskybathynella Serban and Leclerc, 1984 (Syncarida, Bathynellacea, Bathynellidae) in the Iberian Peninsula: three new species. Jounal of Natural History, 41(45-48): 2817-2841. http://dx.doi.org/10.1080/00222930701770760

Camacho, A. I., Dorda, B. A. & Rey, I., 2011. Identifying Cryptic speciation across groudwater populations: first COI sequences of Bathynellidae (Crustacea, Syncarida). Graellsia, 67(1): 7-12. http://dx.doi.org/10.3989/graellsia.2011.v67.031

Camacho, A. I., Dorda, B. A. & Rey, I., 2012. Undisclosed taxonomic diversity of Bathynellacea (Malacostraca: Syncarida) in the Iberian Peninsula revealed by molecular data. Journal of Crustacean Biology, 32(5): 816-826. http://dx.doi.org/10.1163/193724012X638473

Camacho, A. I., Dorda, B. A. & Rey, I., 2013 (in press). Old and new taxonomic tools: description of a new genus and two new species of Bathynellidae family from Spain with morphological and molecular characters. Journal of Natural History (in press)

Chenuil, A., 2006. Choosing the right molecular genetic markers for studying biodiversity:from molecular evolution to practical aspects. Genetica, 127: 101-120. http://dx.doi.org/10.1007/s10709-005-2485-1

Costa, F. O., de Waard, J. R., Boutillier, J., Ratnasingham, S., Dooh, R. T., Hajibabaei, M. & Hebert, P. D., 2007. Biological identifications through barcodes: the case of the Crustacea. Canadian Journal of Fisheries and Aquatic Sciences, 64: 272-295. http://dx.doi.org/10.1139/f07-008

Felsenstein, J., 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17: 368-376. http://dx.doi.org/10.1007/BF01734359

Felsenstein, J., 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39: 783-791. http://dx.doi.org/10.2307/2408678

Felsenstein, J. & Kishino, J. H., 1993. Is there something wrong with the bootstrap on phylogenies? A reply to Hillis and Bull. Systematic Biology, 42: 193-200.

Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenoek, R., 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit 1 from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294-299.

Giribet, G., Carranza, S., Bagu-à, J., Riutort, M. & Ribera, C., 1996. First molecular evidence for the existence of a Tardigrada + Arthropoda clade. Molecular Biology and Evolution, 13: 76-84. http://dx.doi.org/10.1093/oxfordjournals.molbev.a025573

Gu, X., Fu, Y. X. & Li, W. H., 1995. Maximum likelihood estimation of the heterogeneity of substitution rate among nucleotide sites. Molecular Biology and Evolution, 12: 546-557.

Hillis, D. M. & Dixon, M. T., 1991. Ribosomal DNA: Molecular Evolution and Phylogenetic Inference. The Quarterly Review of Biology, 66: 411-453. http://dx.doi.org/10.1086/417338

Huelsenbeck, J. P. & Ronquist, F., 2001. MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics, 17: 754-755. http://dx.doi.org/10.1093/bioinformatics/17.8.754

Lefébure, T., Douady, C. J., Gouy, M., Trontelj, P., Briolay, J. & Gibert, J., 2006. Phylogeography of a subterranean amphipod reveals cryptic diversity and dynamic evolution in extreme environments. Molecular Ecology, 15: 1797-1806. http://dx.doi.org/10.1111/j.1365-294X.2006.02888.x

Meier, R., Zhang, G. & Ali, F., 2008. The use of mean instead of smallest interspecific distances exaggerates the size of the "barcoding gap" and leads to misidentification. Systematics Biology, 57: 809-813. http://dx.doi.org/10.1080/10635150802406343

Noodt, W. & Galhano, M. H., 1969. Studien an Crustacea Subterranea (Isopoda, Syncarida, Copepoda) aus dem Norden Portugals. Publicaçoes do Instituto de Zoología "Dr. Augusto Nobre", 107: 9-75.

Posada, D. & Crandall, K. A., 1998. Model test: testing the model of DNA substitution. Bioinformatics, 14: 817-818. http://dx.doi.org/10.1093/bioinformatics/14.9.817

Ronquist, F. & Huelsenbeck, J. P., 2003. MrBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19: 1572-1574. http://dx.doi.org/10.1093/bioinformatics/btg180

Serban, E., 1977. Sur les Bathynellidae (Podophallocarida, Bathynellacea) de l'Italie: Meridiobathynella cf. rouchi Serban, Coineau et Delamare. Travaux de l'Institut de Spèologie ''Émile Racovitza'', 16: 17-35.

Serban, E., 1989a. Taxa nouveaux des bathynellides d'Europe (Bathynellacea, Podophallocarida, Malacostraca). Travaux de l'Institut de Spèologie ''Émile Racovitza'', 28: 3-17.

Serban, E., 1989b. Le système des Gallobathynellines et sur certains rapports entre les péréiopodes 8 des Bathynellidés (Bathynellacea, Podophallocarida, Malacostraca). Miscellanea Speologica Romanica, 1: 121-168.

Serban, E., 2000. Uenobathynella n.g., Parauenobathynella n.g., Morimotobathynella n.g., Nihobathynella n.g. et Paradoxibathynella n.g., Bathynellinae du Japón (Bathynellidae, Bathynellacea, Podophallocarida). Travaux de l'Institut de Spèologie ''Émile Racovitza'', 36: 3-61.

Serban, E. & Leclerc, P., 1984. Cinq taxa nouveaux des Bathynellidés de France (Bathynellacea, Podophallocarida, Malacostraca). Travaux de l'Institut de Spèologie ''Émile Racovitza'', 23: 7-18.

Simon, C. l., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P., 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved PCR primers. Annals of the Entomological Society of America, 87: 51-701

Swofford, D. L., 2002. PAUP*: Phylogeny Analysis Using Parsimony (*and other methods), version 4.0b9. Sinauer Associates Inc., Sunderland, Massachusetts.

Swofford, D. L., Olsen, G. L., Waddell, P. J. & Hillis, D. M., 1996. Phylogenetic inference. In: D. M. Hillis, C. Moritz & B. K. Mable (eds.). Molecular Systematics, second edition. Sinauer Associates, Sunderland, Massachussetts: 407-514.

Tamura, K., Dudley, J., Nei, M. & Kumar, S., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology & Evolution, 24: 1596-1599. http://dx.doi.org/10.1093/molbev/msm092

Walsh, P., Metzger, D. & Higuchi, R., 1991. Chelex 100 as a medium for simple extraction of DNA for PCRbased typing from forensic material. Biotechniques, 10: 506-513.

Whiting, M. F., Carpenter, J. C., Wheeler, Q. D. & Wheeler, W. C., 1997. The Strepsiptera problem: phylogeny of the holometabolous Insect orders inferred from 18S and 28S ribosomal DNA sequences and morphology. Systematic Biology, 46: 1-68. d Yang, Z., 1994. Estimating the pattern of nucleotide substitution. Journal of Molecular Evolution, 39: 105-111.

Zwickl, D. J., 2006. Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Unpublished Ph.D. dissertation, University of Texas at Austin. Zwickl, D. J. & Balhoff, J., 2006. GARLIv0.954.