Graellsia, Vol 74, No 1 (2018)

Pipunculidae (Diptera) del Parque Nacional de la Caldera de Taburiente, La Palma (Islas Canarias, España) — Investigando la variabilidad morfológica y molecular de una nueva especie de moscas cabezonas


https://doi.org/10.3989/graellsia.2018.v74.191

Christian Kehlmaier
Senckenberg Natural History Collections Dresden, Museum of Zoology, Alemania
orcid http://orcid.org/0000-0001-9622-0566

Miguel Ángel Alonso-Zarazaga
Museo Nacional de Ciencias Naturales, Departamento de Biodiversidad y Biología Evolutiva, España
orcid http://orcid.org/0000-0002-6991-0980

Resumen


Este trabajo es el resultado del proyecto “Inventario y estudio de la fauna invertebrada del Parque Nacional de la Caldera de Taburiente” en la isla de La Palma, Islas Canarias. De las cuatro especies recogidas, se describe Chalarus guanche sp. nov. que se registra asimismo de Madeira, y Tomosvaryella freidbergi De Meyer, 1995 y T. parakuthyi De Meyer, 1995 son nuevos registros para La Palma. Se estudia la variabilidad morfológica y molecular de C. guanche sp. nov. y se discute la presencia de variación intragenómica en el ADNr ITS2.

Palabras clave


Diptera; Pipunculidae; Chalarus guanche sp. nov.; Parque Nacional de Caldera de Taburiente; La Palma; Islas Canarias; nueva especie; DNA-barcoding; variabilidad intragenómica; COI; ITS2

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Referencias


Astrin, J. & Stüben, P., 2008. Phylogeny in cryptic weevils: molecules, morphology and new genera of western Palaearctic Cryptorhynchinae (Coleoptera: Curculionidae). Invertebrate Systematics, 22(5): 503–522. https://doi.org/10.1071/IS07057

Beebe, N. W. & Saul, A., 1995. Discrimination of all members of the Anopheles punctulatus complex by polymerase chain reaction-restriction fragment length polymorphism analysis. The American Journal of Tropical Medicine and Hygiene, 53: 478–481. https://doi.org/10.4269/ajtmh.1995.53.478 PMid:7485705

Bourke, B. P., Porangaba Oliveira, T., Suesdek, L., Sterlino Bergo, E. & Mureb Sallum, M. A., 2013. A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae). Parasites & Vectors, 6: 111, 16 pp.

Brown, D. D., Wensink, P. C. & Jordan, E., 1972. Xenopus laevis and Xenopus mulleri: the evolution of tandem genes. Journal of Molecular Biology, 63: 57–73. https://doi.org/10.1016/0022-2836(72)90521-9

De Meyer, M., 1995. The pipunculid flies of Israel and the Sinai (Insecta, Diptera, Pipunculidae). Spixiana, 18: 283–319.

De Meyer, M., Földvári, M. & Báez, M., 2001. The Pipunculidae (Diptera) fauna of the Canary Islands and Madeira. Bulletin et Annales de la Société Royale belge d'Entomologie, [2000], 136: 144–152.

Domingo-Quero, T., Alonso-Zarazaga, M.A., Sánchez- Ruiz, A., Araujo Armero, R., Navas Sánchez, A., Sánchez Moreno, S., García Becerra, R., Nebreda, M., Sánchez Ruiz, M., Fontal-Cazalla, F. & Nieves-Aldrey, J.L., 2003. Inventariando la biodiversidad en el Parque Nacional de la Caldera de Taburiente (La Palma, Islas Canarias, Espa-a): novedades científicas. Graellsia, 59(2–3): 45–68. https://doi.org/10.3989/graellsia.2003.v59.i2-3.235

Földvári, M. & De Meyer, M., 1999. Revision of Central and West European Tomosvaryella Aczél species (Diptera, Pipunculidae). Acta Zoologica Scientiarum Hungaricae, 45: 299–334.

Fregel, R., Pestano, J., Arnay, M., Cabrera, V. M., Larruga, J. M. & González, A. M., 2009. The maternal aborigine colonization of La Palma (Canary Islands). European Journal of Human Genetics, 17(10): 1314–1324. https://doi.org/10.1038/ejhg.2009.46 PMid:19337312 PMCid:PMC2986650

Hall, T. A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95–98.

Harris, D. J. & Crandall, K. A., 2000. Intragenomic variation within ITS1 and ITS2 of freshwater crayfishes (Decapoda: Cambaridae): implications for phylogenetic and microsatellite studies. Molecular Biology and Evolution, 17(2): 284–291. https://doi.org/10.1093/oxfordjournals.molbev.a026308 PMid:10677851

Hebert P. D. N., Cywinska A., Ball S. L. & deWaard J. R., 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B, Biological Sciences, 270: 313–321. https://doi.org/10.1098/rspb.2002.2218 PMid:12614582 PMCid:PMC1691236

Hoy, M. S. & Rodriguez, R. J., 2013. Intragenomic sequence variation at the ITS1–ITS2 region and at the 18S and 28S nuclear ribosomal DNA genes of the New Zealand mud snail, Potamopyrgus antipodarum (Hydrobiidae: Mollusca). Journal of Molluscan Studies, 79: 205–217. https://doi.org/10.1093/mollus/eyt016

Kehlmaier, C., 2005. Taxonomic revision of European Eudorylini. Verhandlungen des Naturwissenschaftlichen Vereins in Hamburg (Nova Folia), 41: 45–353.

Kehlmaier, C. & Assmann, T., 2008. The European species of Chalarus Walker, 1834 revisited (Diptera: Pipunculidae). Zootaxa, 1936: 1–39.

Kehlmaier, C. & Assmann, T., 2010. Molecular analysis meets morphology-based systematics—a synthetic approach for Chalarinae (Insecta: Diptera: Pipunculidae). Systematic Entomology, 35: 181–195. https://doi.org/10.1111/j.1365-3113.2009.00500.x

Li, C. & Wilkerson, R. C., 2007. Intragenomic rDNA ITS2 variation in the neotropical Anopheles (Nyssorhynchus) albitarsis complex (Diptera: Culicidae). Journal of Heredity, 98(1): 51–59. https://doi.org/10.1093/jhered/esl037 PMid:17158469

Lindner, D. L. & Banik, M. T., 2011. Intragenomic variation in the ITS rDNA region obscures phylogenetic relationships and inflates estimates of operational taxonomic units in genus Laetiporus. Mycologia, 103(4): 731–740. https://doi.org/10.3852/10-331 PMid:21289107

O'Donnell, K. & Cigelnik, E., 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution, 7(1): 103–116. https://doi.org/10.1006/mpev.1996.0376 PMid:9007025

Odorico, D. M. & Miller, D. J., 1997. Variation in the ribosomal internal transcribed spacers and 5.8s rDNA among five species of Acropora (Cnidaria; Scleractinia): patterns of variation consistent with reticulate evolution. Molecular Biology and Evolution, 14(5): 465-473. https://doi.org/10.1093/oxfordjournals.molbev.a025783 PMid:9159924

Padial, J. M., Miralles, A., De la Riva, I. & Vences, M., 2010. The integrative future of taxonomy. Frontiers in Zoology, 2010:7, 16 pp.

Rafael, J. A. & Skevington, J. H., 2010. Pipunculidae (big-headed flies). In: B.V. Brown, A. Borkent, J.M. Cumming, D.M. Wood, N.E. Woodley & M.A. Zumbado (eds.). Manual of Central American Diptera. Volume 2. NRC Research Press. Ottawa: 793–803.

Schlick-Steiner B. C., Steiner, F. M., Seifert, B., Stauffer, C., Christian, E. & Crozier, R. H., 2010. Integrative taxonomy: a multisource approach to exploring biodiversity. Annual Review of Entomology 2010, 55: 421–438. https://doi.org/10.1146/annurev-ento-112408-085432 PMid:19737081

Shapoval, N. A. & Lukhtanov, V. A., 2015. Intragenomic variations of multicopy ITS2 marker in Agrodiaetus blue butterflies (Lepidoptera, Lycaenidae). Comparative Cytogenetics, 9(4): 483–497. https://doi.org/10.3897/CompCytogen.v9i4.5429 PMid:26753069 PMCid:PMC4698565

Stewart, F. J. & Cavanaugh, C. M., 2007. Intragenomic variation and evolution of the internal transcribed spacer of the rRNA operon in Bacteria. Journal of Molecular Evolution, 65: 44–67. https://doi.org/10.1007/s00239-006-0235-3 PMid:17568983

Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30: 2725–2729. https://doi.org/10.1093/molbev/mst197 PMid:24132122 PMCid:PMC3840312

Vesgueiro, F. T., Demari-Silva, B., Malafronte, R. dos S., Sallum, M. A. M. & Marrelli, M. T., 2011. Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae). Memórias do Instituto Oswaldo Cruz, 106(1): 1–8. https://doi.org/10.1590/S0074-02762011000100001

Weitemier, K., Straub, S. C. K., Fishbein, M. & Liston, A., 2015. Intragenomic polymorphisms among high-copy loci: a genus-wide study of nuclear ribosomal DNA in Asclepias (Apocynaceae). PeerJ, 3: e718, 23 pp.

Wörheide, G., Nichols, S. A. & Goldberg, J., 2004. Intragenomic variation of the rDNA internal transcribed spacers in sponges (Phylum Porifera): implications for phylogenetic studies. Molecular Phylogenetics and Evolution, 33: 816–830. https://doi.org/10.1016/j.ympev.2004.07.005 PMid:15522806

Yeates, D. K., Seago, A., Nelson, L., Cameron, S. L., Joseph, L. & Trueman, J. W. H., 2011. Integrative taxonomy, or iterative taxonomy? Systematic Entomology, 36(2): 209– 217. https://doi.org/10.1111/j.1365-3113.2010.00558.x




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